New Genotype G3 P[8] of Rotavirus Identified in a Mexican Gastroenteric Rabbit
Emmanuel Reynoso-Utrera,
Linda Guiliana Bautista-Gómez,
Salvador Fonseca-Coronado,
Juan Diego Pérez-de la Rosa,
Valeria Jazmín Rodríguez-Villavicencio,
Camilo Romero-Núñez,
Ariadna Flores-Ortega,
Pedro Abel Hernández-García,
José Simón Martínez-Castañeda
Affiliations
Emmanuel Reynoso-Utrera
Laboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, Mexico
Linda Guiliana Bautista-Gómez
Laboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, Mexico
Salvador Fonseca-Coronado
Laboratorio de Investigación en Inmunología y Salud Pública, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, San Sebastian Xhala 54714, Mexico
Juan Diego Pérez-de la Rosa
Laboratorio de Diagnóstico Molecular JGG & RNL, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, Mexico
Valeria Jazmín Rodríguez-Villavicencio
Laboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, Mexico
Camilo Romero-Núñez
Hospital Veterinario DERMAVET, Ciudad de México 09510, Mexico
Ariadna Flores-Ortega
Laboratorio de Biotecnología, Biología Molecular y Genética, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, Mexico
Pedro Abel Hernández-García
Laboratorio Multidisciplinario en Investigación, Centro Universitario UAEM Amecameca, Universidad Autónoma del Estado de México, Amecameca 56900, Mexico
José Simón Martínez-Castañeda
Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca 50000, Mexico
Rotavirus species A (RVA) is a major cause of acute viral gastroenteritis in young humans and diverse animal species. The study of the genetic characteristics of RVAs that infect rabbits (Oryctolagus cuniculus) (lapine strain [LRV]) has been limited, and, to date, the most common and epidemiologically important combinations of G/P genotypes in rabbits have been reported to be G3 P[14] and G3 P[22]. In this study, a rotavirus species A detected from an outbreak of enteritis in a Mexican commercial rabbitry was genotypically characterized. Based on sequence and phylogenetic analysis of the VP7 and VP4 genes, the strain identified in this study (C-3/15) demonstrated a G3 P[8] genotype of rotavirus, which had not previously been reported in rabbits. Moreover, both genes were closely related to human, not lapine, rotaviruses. The G3 genotype has been reported in a wide variety of hosts, including humans and rabbits, whereas the P[8] genotype has only been reported in humans. Because this combination of genotypes has never been identified in rabbits, it is proposed that the finding presented here is possibly the result of an interspecies transmission event. This is the first work to study the molecular characteristics of rotaviruses in rabbits in Mexico, as well as the identification of human G3 and P[8] genotypes in a rabbit with enteric disease.
