Effects of Crocin on Oxidative Stress Indices in Rat Brain Demylated with Ethidium Bromide

Abstract

Background and purpose: The purpose of this study was to investigate the effects of crocin (Cr) on brain oxidative stress indices in animal model demyelinated with intracerebroventricular ethidium bromide (EB). Materials and methods: In an interventional study, female Wistar rats were randomly divided into 7 groups. Crocin administration was done 1 day after EB injection for 21 days. The values ​​for Glutathione peroxidase (GPx), Superoxide dismutase (SOD), and Malondialdehyde (MDA) were measured before and after receiving Cr and analyzed using Paired t test and ANOVA. Results: The levels of GPx, SOD, and MDA significantly reduced in the group that received Cr100 mg/kg (P<0.05). The effects of crocin were dose-dependent and other doses did not demonstrate significant effects on oxidative stress indices (P< 0.05). Conclusion: The antioxidant effects of Crocin were dose-dependent, and it could be used as a strong antioxidant in management of patients with multiple sclerosis.

Keywords

• demyelination
• oxidative stress
• crocin
• malondialdehyde
• superoxide dismutase