Age and gender related paraoxonase I gene polymorphisms rs662 and rs854560 and serum activity in subjects with metabolic syndrome in Fars ethnic group

Abstract

Background: The prevalence of Metabolic Syndrome (MetS) is different among different populations. The human Paraoxonase (PON) genes have been less reported in the literature. So it is a need of hour to study PON. Aim and Objectives: To determine the Paraoxonase I (PON1) gene polymorphisms and its paraoxonase activity in subjects with and without Metabolic Syndrome (MetS). Material and Methods: Determination of the PON1 gene polymorphisms were done using Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) analysis. Results: The MM, LM, and LL genotype frequencies of the PON1- L55M polymorphism were 2.5, 37.5 and 60%; and 27.6, 34.5 and 37.9% and; 19.4, 50 and 30.6% and 17.2, 58.6 and 34.2% in females and males with and without MetS, respectively. Females with L allele of L55M polymorphism had a nearly 14.86 -fold risk of developing MetS. Conclusion: Our study suggested that the decrease of PON1 enzymatic activity in both genders is an important finding, but the L55M genotype and L allele in females with MetS are more important risk factors of MetS than PON1 Q192R polymorphism. This difference in L55M among females may make them more vulnerable than males to MetS.

Keywords

• age
• gender
• paraoxonase i
• metabolic syndrome
• fars