International Journal of Nanomedicine (Dec 2021)

Platelet-Tumor Cell Hybrid Membrane-Camouflaged Nanoparticles for Enhancing Therapy Efficacy in Glioma

  • Wu L,
  • Li Q,
  • Deng J,
  • Shen J,
  • Xu W,
  • Yang W,
  • Chen B,
  • Du Y,
  • Zhang W,
  • Ge F,
  • Lei S,
  • Li K,
  • Wang Z

Journal volume & issue
Vol. Volume 16
pp. 8433 – 8446


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Lingling Wu, 1,* Qin Li, 2,* Junjie Deng, 3, 4 Jinglan Shen, 2 Weide Xu, 3 Wei Yang, 5 Bingyu Chen, 2 Yaoqiang Du, 2 Wei Zhang, 6 Feihang Ge, 6 Siyun Lei, 2 Kaiqiang Li, 2 Zhen Wang 2 1Women’s Hospital, School of Medicine, Zhejiang University, Hangzhou, 310014, People’s Republic of China; 2Department of Transfusion Medicine, Allergy Center, Ministry of Education Key Laboratory of Laboratory Medicine, Zhejiang Provincial People’s Hospital People’s Hospital of Hangzhou Medical College, Hangzhou, 310014, People’s Republic of China; 3Zhejiang Engineering Research Center for Tissue Repair Materials, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325000, People’s Republic of China; 4Oujiang Laboratory, Wenzhou, Zhejiang, 325000, People’s Republic of China; 5Department of Biophysics, and Department of Neurosurgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People’s Republic of China; 6Hangzhou Chinese Academy of Sciences-Hangzhou Medical College Advanced Medical Technology Institute, Hangzhou, 310014, People’s Republic of China*These authors contributed equally to this workCorrespondence: Kaiqiang Li; Zhen WangZhejiang Provincial People’s Hospital, Hangzhou, People’s Republic of ChinaTel +86 0571-87666666Fax +86 0571-85131448Email [email protected]; [email protected]: Cell membrane-camouflaged nanoparticles (NPs) are drawing increasing attention because their surfaces acquire some characteristics of the cell membranes, making them a unique class of biomimetic materials for diverse applications. Modification of cell membrane or combination of different types of membranes can enhance their functionality.Methods: We prepared platelet and tumor cell membrane camouflaged β-mangostin-loaded NPs, which were synthesized with platelet–C6 hybrid biomimetic coating, poly(lactic-co-glycolic acid), and β-mangostin (β-PCNPs). Then, we evaluated their targeting ability and anticancer activity against glioma in vitro and in vivo.Results: Biomimetic coating enhanced active drug targeting and immune escape properties of nanocarrier in C6 and THP-1 cells, respectively, which improved their cytotoxicity. β-PCNPs were characterized to study the inherent properties of both source cells. Compared with bare β-NPs, β-PCNPs exhibited high tumor-targeting capability and induced apoptosis of C6 cells in vitro. Similarly, intravenous administration of drug through β-PCNPs resulted in enhanced tumor-targeting and exhibited excellent rate of inhibition of glioma tumor growth in mice. Moreover, the blood circulation time of drug in mice in the β-PCNP group was markedly prolonged and these mice exhibited better outcome than those in the β-NP group.Conclusion: These results provide a new strategy of utilizing PCNPs as carriers for drug delivery, which improves the targeting efficiency and therapeutic efficacy of chemotherapeutic agents for glioma therapy.Keywords: glioma, hybrid membrane, biomimetic, β-mangostin, targeted delivery, anticancer