Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

Jie Yang; Derrick Cumberbatch; Samuel Centanni; Shu-qun Shi; Danny Winder; Donna Webb; Carl Hirschie Johnson

doi:10.1038/ncomms13268

Nature Communications (Oct 2016)

Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing

Jie Yang,
Derrick Cumberbatch,
Samuel Centanni,
Shu-qun Shi,
Danny Winder,
Donna Webb,
Carl Hirschie Johnson

Affiliations

Jie Yang: Department of Biological Sciences, Vanderbilt University
Derrick Cumberbatch: Department of Biological Sciences, Vanderbilt University
Samuel Centanni: Department of Molecular Physiology and Biophysics, Vanderbilt University
Shu-qun Shi: Department of Biological Sciences, Vanderbilt University
Danny Winder: Department of Molecular Physiology and Biophysics, Vanderbilt University
Donna Webb: Department of Biological Sciences, Vanderbilt University
Carl Hirschie Johnson: Department of Biological Sciences, Vanderbilt University

DOI: https://doi.org/10.1038/ncomms13268
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 10

Abstract

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The coupling of optogenetics with fluorescent Ca2+ sensors is confounded by sensitivity of optogenetic probes to light used to excite the sensors. Here the authors develop a Ca2+ sensor based on bioluminescence resonance energy transfer (BRET) that monitors Ca2+fluxes in darkness without excitation.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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