Typhim vi immunization assists to discriminate primary antibody responses in hematological malignancies
J. Ochoa-Grullón,
C. Orte,
A. Rodríguez de la Peña,
K. Guevara-Hoyer,
G. Cordero Torres,
M. Fernández-Arquero,
I. Serrano-García,
M.J. Recio,
R. Pérez de Diego,
S. Sánchez-Ramón
Affiliations
J. Ochoa-Grullón
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain; Department of Immunology, Ophthalmology and ENT, School of Medicine, Complutense University School of Medicine, Madrid, Spain; Immunodeficiency Interdepartmental Group (GIID), Madrid, Spain
C. Orte
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain
A. Rodríguez de la Peña
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain
K. Guevara-Hoyer
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain; Department of Immunology, Ophthalmology and ENT, School of Medicine, Complutense University School of Medicine, Madrid, Spain; Immunodeficiency Interdepartmental Group (GIID), Madrid, Spain
G. Cordero Torres
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain
M. Fernández-Arquero
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain
I. Serrano-García
Department of Epidemiology and Preventive Medicine, Hospital Clínico San Carlos, Madrid, Spain
M.J. Recio
Immunodeficiency Interdepartmental Group (GIID), Madrid, Spain; Laboratory of Immunogenetics of Human Diseases, IdiPAZ Institute for Health Research, Madrid, Spain
R. Pérez de Diego
Immunodeficiency Interdepartmental Group (GIID), Madrid, Spain; Laboratory of Immunogenetics of Human Diseases, IdiPAZ Institute for Health Research, Madrid, Spain
S. Sánchez-Ramón
Department of Immunology, IML and IdSSC, Hospital Clínico San Carlos, Madrid, Spain; Department of Immunology, Ophthalmology and ENT, School of Medicine, Complutense University School of Medicine, Madrid, Spain; Immunodeficiency Interdepartmental Group (GIID), Madrid, Spain; Corresponding author
ABSTRACT: Assessment of specific antibody (Ab) production to polysaccharide antigens is clinically relevant, identifying patients at risk for infection by encapsulated bacteria and thus enabling a more rigorous selection of patients that can benefit of immunoglobulin replacement therapy. Classically, the gold-standard test is the measurement of antibody production to pure polysaccharide pneumococcal (PPV) immunization. Several factors, including introduction of conjugate vaccination schedule, serotyping analysis, high baseline Ab levels, have hindered the evaluation of polysaccharide antigens. This is even more difficult in secondary immunodeficiencies (SID), where patients can show secondary responses despite lack of primary antibody responses and present with recurrent or severe infections. Assessment of specific Ab production to pure Salmonella typhi Vi polysaccharide (TV) immunization has been proposed as a complementary test to PPV, given its low seroprevalence. To set the optimal cut-off value for PPV and TV response in SID, we tested different biostatistical methodologies, including ROC analysis, Youden index, Union index and Closest-topleft in a cohort of 42 SID patients and 24 healthy controls. The statistically chosen cut-offs value pre-post TV Ab ratio was ≥5, (sensitivity of 90%, specificity of 100%) and a postvaccination TV concentration of 28.5 U/mL (sensitivity of 90%, specificity of 95%), showing relevant clinical correlate.