Nature Communications (May 2022)
Multivalent interactions essential for lentiviral integrase function
- Allison Ballandras-Colas,
- Vidya Chivukula,
- Dominika T. Gruszka,
- Zelin Shan,
- Parmit K. Singh,
- Valerie E. Pye,
- Rebecca K. McLean,
- Gregory J. Bedwell,
- Wen Li,
- Andrea Nans,
- Nicola J. Cook,
- Hind J. Fadel,
- Eric M. Poeschla,
- David J. Griffiths,
- Javier Vargas,
- Ian A. Taylor,
- Dmitry Lyumkis,
- Hasan Yardimci,
- Alan N. Engelman,
- Peter Cherepanov
Affiliations
- Allison Ballandras-Colas
- Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- Vidya Chivukula
- Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- Dominika T. Gruszka
- Single Molecule Imaging of Genome Duplication and Maintenance Laboratory, The Francis Crick Institute
- Zelin Shan
- Laboratory of Genetics, The Salk Institute for Biological Studies
- Parmit K. Singh
- Department of Cancer Immunology & Virology, Dana-Farber Cancer Institute
- Valerie E. Pye
- Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- Rebecca K. McLean
- Moredun Research Institute, Pentlands Science Park, Bush Loan
- Gregory J. Bedwell
- Department of Cancer Immunology & Virology, Dana-Farber Cancer Institute
- Wen Li
- Department of Cancer Immunology & Virology, Dana-Farber Cancer Institute
- Andrea Nans
- Structural Biology Science Technology Platform, The Francis Crick Institute
- Nicola J. Cook
- Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- Hind J. Fadel
- Division of Infectious Diseases, Mayo Clinic
- Eric M. Poeschla
- Division of Infectious Diseases, University of Colorado Anschutz Medical Campus
- David J. Griffiths
- Moredun Research Institute, Pentlands Science Park, Bush Loan
- Javier Vargas
- Departmento de Óptica, Universidad Complutense de Madrid
- Ian A. Taylor
- Macromolecular Structure Laboratory, The Francis Crick Institute
- Dmitry Lyumkis
- Laboratory of Genetics, The Salk Institute for Biological Studies
- Hasan Yardimci
- Single Molecule Imaging of Genome Duplication and Maintenance Laboratory, The Francis Crick Institute
- Alan N. Engelman
- Department of Cancer Immunology & Virology, Dana-Farber Cancer Institute
- Peter Cherepanov
- Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- DOI
- https://doi.org/10.1038/s41467-022-29928-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
The authors determined high-resolution cryo-EM structures of the lentiviral intasome — the nucleoprotein complex that inserts viral DNA into a host chromosome — and show that the architecture comprising 16 integrase subunits is critical for its function.
