Hepatotoxic components and attenuation of polygonimultiflori radix praeparata induced idiosyncratic liver injury
Ren Hongwei,
Pu Weiling,
Wei Jing,
Han Lifeng,
Fu Zhifei,
Su Yanfang,
Chang Yanxu,
Hu Limin,
Fan Guanwei,
Gao Xiumei
Affiliations
Ren Hongwei
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Pu Weiling
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Wei Jing
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Han Lifeng
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Fu Zhifei
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Su Yanfang
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China
Chang Yanxu
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Hu Limin
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Fan Guanwei
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Gao Xiumei
Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education,Tianjin University of Traditional Chinese Medicine，Tianjin 301617, China
Objective Polygoni Multiflori Radix (PM, He-Shou-Wu), is used as a reputable tonifying traditional Chinese medicine for a long time. However, in recent years, the liver injury caused by PM or its preparations attracted wide attention. This research was to investigate the mechanism, material basis and attenuation of PM induced idiosyncratic liver injury. Methods In this study, we investigated the molecular basis of PM-induced liver injury based on a non-hepatotoxic dose of LPS. A component “knock-out” strategy with TSG (2,3,5,4'-tet‐rahydroxy stilbene-2-O-β-D-glucopyranoside) or AN (anthraquinone) eliminated was used to study the materialbasis for PM-induced liver injury. Finally, some polysaccharides and oligosaccharides were selected to exploretheir toxicity attenuated effects when used compatible with PM. Results In LPS-induced immune stress mice or rats, PM caused immune-idiosyncratic liver injury. After knockout anthraquinones (AN) from PM (PM-AN), the liver injury was significantly reduced and the AN isolated from PM also caused immune-idiosyncratic liver injury.Pretreatment with Cistanchedeserticola polysaccharide (CDP), Alginate oligosaccharide (AO), Chitosan oligosac‐charide (CO) could attenuate the PM-induced liver injury and inhibit the expression of various cytokines. Conclusion The immune-idiosyncratic constitution is an important factor leading to the liver injury of PM. Anthraqui‐none is a major material basis of PM-induced liver injury. Polysaccharides or oligosaccharides could attenuate PMinduced liver injury, which was related with immune-modulatory effects.
