Cell Reports (Feb 2023)

Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays

  • Maria Rosito,
  • Caterina Sanchini,
  • Giorgio Gosti,
  • Manuela Moreno,
  • Simone De Panfilis,
  • Maria Giubettini,
  • Doriana Debellis,
  • Federico Catalano,
  • Giovanna Peruzzi,
  • Roberto Marotta,
  • Alessia Indrieri,
  • Elvira De Leonibus,
  • Maria Egle De Stefano,
  • Davide Ragozzino,
  • Giancarlo Ruocco,
  • Silvia Di Angelantonio,
  • Francesca Bartolini

Journal volume & issue
Vol. 42, no. 2
p. 112104


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Summary: Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.