Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy

Gang Ma; Hong Zhang; Jianhua Guo; Xiaodan Zeng; Xiaoqian Hu; Wenying Hao

doi:10.1155/2014/285806

Journal of Spectroscopy (Jan 2014)

Assessment of the Inhibitory Effect of Rifampicin on Amyloid Formation of Hen Egg White Lysozyme: Thioflavin T Fluorescence Assay versus FTIR Difference Spectroscopy

Gang Ma,
Hong Zhang,
Jianhua Guo,
Xiaodan Zeng,
Xiaoqian Hu,
Wenying Hao

Affiliations

Gang Ma: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
Hong Zhang: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
Jianhua Guo: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
Xiaodan Zeng: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
Xiaoqian Hu: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China
Wenying Hao: Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China

DOI: https://doi.org/10.1155/2014/285806
Journal volume & issue: Vol. 2014

Abstract

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The inhibitory effect of rifampicin on the amyloid formation of hen egg white lysozyme was assessed with both Thioflavin T (ThT) fluorescence assay and Fourier transform infrared (FTIR) difference spectroscopy. We reveal that ThT fluorescence assay gives a false positive result due to rifampicin interference, while FTIR difference spectroscopy provides a reliable assessment. With FTIR, we show that rifampicin only has marginally inhibitory effect. We then propose that FTIR difference spectroscopy can potentially be a convenient method for inhibitor screening in amyloid study.

Published in Journal of Spectroscopy

ISSN: 2314-4920 (Print); 2314-4939 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Physics: Optics. Light
Website: https://onlinelibrary.wiley.com/journal/9169

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