Metabolism of glycerolipids: V. Metabolism of phosphatidic acid

Abstract

Monoacyl glycerol phosphate is acylated more rapidly than glycerol-3-phosphate by microsomal preparations from rat or guinea pig liver.Specifically labeled diacyl glycerol phosphate derivatives were prepared. Only the 2-labeled derivative produced radioactive fatty acids when treated with Crotalus adamanteus venom, indicating that the phospholipase can hydrolyze diacyl glycerol phosphate and that the reaction is specific for the 2-ester.When glycerol phosphate was acylated, linoleate and stearate were incorporated at both the 1- and 2-positions. The results suggest that the specificities of the acyl transferase reactions leading to diacyl glycerol phosphate are not adequate to provide the pattern of fatty acids that is known to occur in glycerolipids in nature.