Assessment Of Differante Techniques For Oocyte Collection For Ivf In The Camel (Camelus dromedarius)

Abstract

The aims of the present study were to compare between the harvested methods of oocytes of she camel ovaries and their effect on the numbers of oocytes collection, Grad of oocytes and ratio of maturation oocytes from AL-Najaf slaughterhouse from December 2017 to January 2018 in rutting season . The oocytes numbers, graded of oocytes and the rate of maturation oocytes. The collection of oocytes was done by aspiration, slicing and slashing .One hundred ninety quality oocytes (190) were recovered from 90 ovaries from she camel at age (4-7) years. The study revealed that slicing method yielded significantly different (p<0.05) number of oocytes from the others methods as 77 oocytes distribution Grad A, B and C 25 (32.4%), 30 (38.9%), 22 (28.7%), respectively. While the aspiration method yielded 54 oocytes distribution Grad A, B and C 22(40.7%), 18(33.3%), 14(25.9%), respectively. The slashing method yielded 59 oocytes distributed grad A, B and C 23(38.9%), 20(33.9%), 16(27.11%).The study appearance the aspiration method was better than the slashing and slicing method which obtained higher quality oocytes maturation percentages and significant different( p<0.05) compared to the other methods. From the forty oocytes cultured in TCM- 199, 18 oocytes showed cumulus expansion with cytoplasmic density and first polar body, The percentages 45% (18/40 mature oocyte). The slashing method collection culture 43 oocytes 15 oocytes showed cumulus expansion and cytoplasm density and first polar body the percentage 34% (15/43). Slicing method collection in same medium, out of 55 oocytes cultured only 11 oocytes showed cumulus expansion with cytoplasm density and first polar body. The percentage was 20% (11/55). There was a significant difference (p<0.01) between different collected method.

Keywords

• Camelus Dromedarius
• Oocyte Collection Techniques
• Oocytes
• Fertilize Camel Mature
• Ivf