Molecular study of Oestrus ovis larvae infesting in sheep in Baghdad city, Iraq

Abstract

The aim of this study is to detect the 28S (rRNA) gene sequences of Oestrus ovis larvae by conventional polymerase chain reaction and to compare their genetic relatedness utilizing phylogenetic analysis. Fifty larvae were collected from sheep for DNA extraction after slaughtering during the period from the beginning of February until the end of April 2019 in Baghdad city. PCR product appeared as the band size 950 bp. Ten of the product PCR were selected for sequence analysis to obtain the partial nucleotides 28S (rRNA) gene. After that the sequence were recorded in National Center for Biotechnology Information (NCBI) with ID NO. (MT875427, MT875428, MT875429, MT875430, MT875431, MT875432, MT875433, MT875434, MT875435, MT875436) for O. ovis larvae. Then, compared these accession number with another global registered in NCBI by using phylogenetic tree examination which show NCBI-BLAST homology sequence identity between them, and these results were confirmed 99% identity with Spain and Brazil isolates and 98% with Italy.

Keywords

• oestrus ovis
• pcr
• sheep