Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections

S. Corbellini; E. Scaltriti; G. Piccinelli; F. Gurrieri; M. Mascherpa; G. Boroni; C. Amolini; A. Caruso; M.A. De Francesco

Journal of Global Antimicrobial Resistance (Mar 2022)

Genomic characterisation of Escherichia coli isolates co-producing NDM-5 and OXA-1 from hospitalised patients with invasive infections

S. Corbellini,
E. Scaltriti,
G. Piccinelli,
F. Gurrieri,
M. Mascherpa,
G. Boroni,
C. Amolini,
A. Caruso,
M.A. De Francesco

Affiliations

S. Corbellini: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy
E. Scaltriti: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Parma, Italy
G. Piccinelli: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy
F. Gurrieri: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy
M. Mascherpa: Department of Obstetrics and Gynecology, University of Brescia–ASST Spedali Civili, Brescia, Italy
G. Boroni: Pediatric Surgery Unit, ASST Spedali Civili of Brescia, Brescia, Italy
C. Amolini: Medicine 1 Unit Geriatric Section, Montichiari Hospital, ASST–Spedali Civili of Brescia, Brescia, Italy
A. Caruso: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy
M.A. De Francesco: Institute of Microbiology, Department of Molecular and Translational Medicine, University of Brescia–ASST Spedali Civili, Brescia, Italy; Corresponding author. Mailing address: Institute of Microbiology, Department of Molecular and Translational Medicine, P. le Spedali Civili 1, 25123 Brescia, Italy. Tel.: +39 030 399 5860; fax: +39 030 399 6071.

Journal volume & issue: Vol. 28
pp. 136 – 139

Abstract

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ABSTRACT: Objectives: Carbapenems are one of the last-report therapeutic choices to treat infections due to multidrug-resistant (MDR) micro-organisms. For this reason, the spread of carbapenemase-producing Enterobacteriaceae represents a serious health-public problem. Here we describe isolates co-producing blaNDM-5 and blaOXA-1. Methods: Three Escherichia coli isolates obtained from patients with invasive infections were analysed by phenotypic antibiotic susceptibility testing and whole-genome sequencing (WGS). Results: All of the isolates were resistant to carbapenems, most β-lactam antibiotics, piperacillin/tazobactam, amoxicillin/clavulanic acid and ciprofloxacin, remaining susceptible to amikacin, fosfomycin, colistin and tigecycline. The isolates belonged to sequence types ST44, ST405 and ST167 and co-harboured the blaNDM-5 and blaOXA-1 genes. Two of the isolates also harboured extended-spectrum β-lactamase (ESBL) genes (blaCTX-M-15 and blaTEM-1b). The blaNDM-5 gene was probably carried chromosomally even if different plasmids were identified. Various virulence genes were also identified. Conclusion: Our results highlight that continuous surveillance is essential to monitor the spread of clinically important MDR pathogens.

Published in Journal of Global Antimicrobial Resistance

ISSN: 2213-7165 (Print); 2213-7173 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology
Website: https://www.journals.elsevier.com/journal-of-global-antimicrobial-resistance

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