Evaluation of relationship between endothelial function and vascular-platelet hemostasis in patients with a good compensation of type 2 diabetes mellitus and hypertension

Abstract

The aim of research. The aim of this study is to determine the relationships between endothelial dysfunction, the degree of renal function damage, lipidemic profile and morphological changes of vascular-platelet hemostasis in patients with good compensation of type 2 diabetes mellitus and arterial hypertension. Materials and methods. In 69 patients with good glycemic compensation using clinical, laboratory, morphological methods and correlational analysis association between endothelial dysfunction, degree of renal function damage, lipidemic profile and morphological changes of vascular-platelet hemostasis have been identified. Results. Results of correlation analysis showed the presence of credible negative relation between the content of inactivated platelets and concentrations of endothelin-1 (r=-0.53; p<0.05), von Willebrand factor (r=-0.68; p<0.05) and thrombomodulin (r=-0.55; p<0.05). The largest number and strength of correlations between morphological indicators of intravascular platelet activation and studied clinical and laboratory parameters were observed with admiration to the activated and aggregated forms of cells. In particular, elevated levels of activated platelets in patients of this contingent had reliable high degree of direct correlation with the content of glycated hemoglobin (r=+0.79), systolic blood pressure (r=+0.63), the concentration of endothelial dysfunction markers, including the level of albuminuria (r=+0.73), as well as with the values of total cholesterol (r=+0.64) and low density lipoprotein cholesterol (r=+0.79). Content of aggregated platelets statistically significantly positively correlated with the level of glycated hemoglobin (r=+0.73), all markers of endothelial dysfunction, urea concentration (r=+0.51) and creatinine (r=+0.58). In contrast, the relative content of degranulated platelet establish a reliable correlation only with the levels of endothelin-1 (r=+0.75) and thrombomodulin (r=+0.62). On the level of endothelin-1 and thrombomodulin concentration positive linear correlation was found, but alpha-granules located in shut inverse correlation not only with endothelin-1 (r=-0.57; p<0.05), as well as with the concentration of glycated hemoglobin (r=-0.76; p<0.05) and with dyslipidemic changes. Increased platelet aggregation activity in patients with good glycemic compensation, which was manifested in a twofold increase in the content of circulating small units was significantly correlated with the level of glycated hemoglobin (r=+0.66) and the values of systolic blood pressure (r=+0.58), as well as concentrations of markers of endothelial dysfunction and renal function, but did not have any significant relationships with blood lipids in patients of this contingent. Conclusion. It has been established that in the good glycemic control morphological criteria of platelet hemostasis damage clearly correlated with markers of endothelial dysfunction, but did not show significant relationships with indicators of renal function and blood lipid profile.

Keywords

• Diabetes Mellitus Type 2
• Hypertension
• Endothelium
• Hemostasis