Nature Communications (May 2019)
Discovery of processive catalysis by an exo-hydrolase with a pocket-shaped active site
- Victor A. Streltsov,
- Sukanya Luang,
- Alys Peisley,
- Joseph N. Varghese,
- James R. Ketudat Cairns,
- Sebastien Fort,
- Marcel Hijnen,
- Igor Tvaroška,
- Ana Ardá,
- Jesús Jiménez-Barbero,
- Mercedes Alfonso-Prieto,
- Carme Rovira,
- Fernanda Mendoza,
- Laura Tiessler-Sala,
- José-Emilio Sánchez-Aparicio,
- Jaime Rodríguez-Guerra,
- José M. Lluch,
- Jean-Didier Maréchal,
- Laura Masgrau,
- Maria Hrmova
Affiliations
- Victor A. Streltsov
- Commonwealth Scientific and Industrial Research Organisation, Materials Science and Engineering
- Sukanya Luang
- School of Agriculture, Food and Wine, University of Adelaide, Waite Campus
- Alys Peisley
- Commonwealth Scientific and Industrial Research Organisation, Materials Science and Engineering
- Joseph N. Varghese
- Commonwealth Scientific and Industrial Research Organisation, Materials Science and Engineering
- James R. Ketudat Cairns
- School of Chemistry and Center for Biomolecular Structure, Function and Application, Suranaree University of Technology
- Sebastien Fort
- University Grenoble Alpes, Centre de Recherches sur les Macromolécules Végétales
- Marcel Hijnen
- GE Healthcare Life Sciences
- Igor Tvaroška
- Institute of Chemistry, Slovak Academy of Sciences
- Ana Ardá
- Centre for Cooperative Research in Biosciences
- Jesús Jiménez-Barbero
- Centre for Cooperative Research in Biosciences
- Mercedes Alfonso-Prieto
- Departament de Química Inorgànica i Orgànica, Universitat de Barcelona
- Carme Rovira
- Departament de Química Inorgànica i Orgànica, Universitat de Barcelona
- Fernanda Mendoza
- Departamento de Ciencias Químicas, Universidad Andrés Bello, Sede Concepción
- Laura Tiessler-Sala
- Departament de Química, Universitat Autònoma de Barcelona
- José-Emilio Sánchez-Aparicio
- Departament de Química, Universitat Autònoma de Barcelona
- Jaime Rodríguez-Guerra
- Departament de Química, Universitat Autònoma de Barcelona
- José M. Lluch
- Departament de Química, Universitat Autònoma de Barcelona
- Jean-Didier Maréchal
- Departament de Química, Universitat Autònoma de Barcelona
- Laura Masgrau
- Departament de Química, Universitat Autònoma de Barcelona
- Maria Hrmova
- School of Agriculture, Food and Wine, University of Adelaide, Waite Campus
- DOI
- https://doi.org/10.1038/s41467-019-09691-z
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 17
Abstract
Enzyme substrates and products often diffuse too rapidly to assess the catalytic implications of these movements. Here, the authors characterise the structural basis of product and substrate diffusion for an exo-hydrolase and discover a substrate-product assisted processive catalytic mechanism.
