PLoS ONE (Jan 2012)

Sequencing by hybridization of long targets.

  • Yu Qin,
  • Tobias M Schneider,
  • Michael P Brenner

DOI
https://doi.org/10.1371/journal.pone.0035819
Journal volume & issue
Vol. 7, no. 5
p. e35819

Abstract

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Sequencing by Hybridization (SBH) reconstructs an n-long target DNA sequence from its biochemically determined l-long subsequences. In the standard approach, the length of a uniformly random sequence that can be unambiguously reconstructed is limited to n = O(2(l)) due to repetitive subsequences causing reconstruction degeneracies. We present a modified sequencing method that overcomes this limitation without the need for different types of biochemical assays and is robust to error.