Design of an Osteoinductive Extracellular Fibronectin Matrix Protein for Bone Tissue Engineering

Abstract

Integrin-mediated cell-matrix interactions play an important role in osteogenesis. Here, we constructed a novel osteoinductive fibronectin matrix protein (oFN) for bone tissue engineering, designed to combine the integrin-binding modules from fibronectin (iFN) and a strong osteoinductive growth factor, bone morphogenetic protein-2. Compared with iFN, the purified oFN matrix protein caused a significant increase in cell adhesion and osteogenic differentiation of pre-osteoblast MC3T3-E1 cells (p < 0.05).

Keywords

• bone morphogenetic protein
• cell adhesion
• cell differentiation
• fibronectin
• MC3T3 cells