Method of Immuno-PCR in diagnostics of bacterial and viral infections

Abstract

Immuno-PCR (I-PCR) combines the capabilities of two modern diagnostic methods of enzyme immunoassay (ELISA) and polymerase chain reaction (PCR), combination these methods causes a 100-10000 fold increase in sensitivity compared to a similar ELISA. The review considers the main variants of I-PCR, gives examples of possible use of the method for early and retrospective diagnosis of various diseases.

Keywords

• immuno-pcr
• enzyme immunoassay
• polymerase chain reaction
• capturing antibodies
• detecting antibodies
• dna label