NIR light-responsive bacteria with live bio-glue coatings for precise colonization in the gut
Meihui Cui,
Tao Sun,
Shubin Li,
Huizhuo Pan,
Jing Liu,
Xinyu Zhang,
Lianyue Li,
Shanshan Li,
Chunyang Wei,
Chengzhuang Yu,
Chun Yang,
Ning Ma,
Binglin Ma,
Shenjunjie Lu,
Jin Chang,
Weiwen Zhang,
Hanjie Wang
Affiliations
Meihui Cui
School of Life Sciences, Tianjin University, Tianjin 300072, China
Tao Sun
Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China; Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China
Shubin Li
Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China
Huizhuo Pan
School of Life Sciences, Tianjin University, Tianjin 300072, China
Jing Liu
School of Life Sciences, Tianjin University, Tianjin 300072, China
Xinyu Zhang
School of Life Sciences, Tianjin University, Tianjin 300072, China
Lianyue Li
School of Life Sciences, Tianjin University, Tianjin 300072, China
Shanshan Li
School of Mechanical Engineering, Hebei University of Technology, Tianjin 300401, China
Chunyang Wei
School of Mechanical Engineering, Hebei University of Technology, Tianjin 300401, China
Chengzhuang Yu
School of Mechanical Engineering, Hebei University of Technology, Tianjin 300401, China
Chun Yang
School of Life Sciences, Tianjin University, Tianjin 300072, China
Ning Ma
School of Life Sciences, Tianjin University, Tianjin 300072, China
Binglin Ma
School of Life Sciences, Tianjin University, Tianjin 300072, China
Shenjunjie Lu
School of Life Sciences, Tianjin University, Tianjin 300072, China
Jin Chang
School of Life Sciences, Tianjin University, Tianjin 300072, China
Weiwen Zhang
Center for Biosafety Research and Strategy, Tianjin University, Tianjin 300072, China; Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China
Hanjie Wang
School of Life Sciences, Tianjin University, Tianjin 300072, China; Corresponding author
Summary: Recombinant bacterial colonization plays an indispensable role in disease prevention, alleviation, and treatment. Successful application mainly depends on whether bacteria can efficiently spatiotemporally colonize the host gut. However, a primary limitation of existing methods is the lack of precise spatiotemporal regulation, resulting in uncontrolled methods that are less effective. Herein, we design upconversion microgels (UCMs) to convert near-infrared light (NIR) into blue light to activate recombinant light-responsive bacteria (Lresb) in vivo, where autocrine “functional cellular glues” made of adhesive proteins assist Lresb inefficiently colonizing the gut. The programmable engineering platform is further developed for the controlled and effective colonization of Escherichia coli Nissle 1917 (EcN) in the gut. The colonizing bacteria effectively alleviate DSS-induced colitis in mice. We anticipate that this approach could facilitate the clinical application of engineered microbial therapeutics to accurately and effectively regulate host health.
