Study on genetic variability of Orobanche populations infecting tobacco in northwest Iran

Abstract

Orobanche spp. is holoparasitic plant, parasitising roots of different crops. Genetic polymorphism was investigated among and within 3 Orobanche species collected from different regions of northwest Iran, using ISSR markers. Out of 34 ISSR primers tested, 20 were found to be polymorphic and produced clear bands .261 discernible bands were generated with 254 (94%) being polymorphic. Among studied species, "O. aegyptiaca" showed 2 unique bands. Clustering algorithm was divided collected Orobanche specimens into 6 main groups. It was obvious that genetic relationships among studied landraces did not have force tendency to associate with their geographic origins. According to AMOVA, 100% of the total variation was partitioned within species. Such variability is important for any attempt to develop resistant host crops against parasite.

Keywords

• amova
• broomrape
• cluster analysis
• genetic diversity
• issr markers