Case Report: Proven Diagnosis of Culture-Negative Chronic Disseminated Candidiasis in a Patient Suffering From Hematological Malignancy: Combined Application of mNGS and CFW Staining

Yanqi Jin; Zhouhan Wang; Chunxia Zhu; Qing Yang; Yingfeng Lu; Xiaopeng Yu; Bao Hong; Xiaojing Wang; Yimin Zhang; Yimin Zhang

doi:10.3389/fmed.2021.627166

Frontiers in Medicine (Feb 2021)

Case Report: Proven Diagnosis of Culture-Negative Chronic Disseminated Candidiasis in a Patient Suffering From Hematological Malignancy: Combined Application of mNGS and CFW Staining

Yanqi Jin,
Zhouhan Wang,
Chunxia Zhu,
Qing Yang,
Yingfeng Lu,
Xiaopeng Yu,
Bao Hong,
Xiaojing Wang,
Yimin Zhang,
Yimin Zhang

Affiliations

Yanqi Jin: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Zhouhan Wang: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Chunxia Zhu: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Qing Yang: Laboratory Department, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Yingfeng Lu: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Xiaopeng Yu: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Bao Hong: Department of Infectious Diseases of Haining Campus, The First Affiliated Hospital, College of Medicine, Zhejiang University, Haining, China
Xiaojing Wang: Department of Infectious Diseases of Haining Campus, The First Affiliated Hospital, College of Medicine, Zhejiang University, Haining, China
Yimin Zhang: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Yimin Zhang: Department of Infectious Diseases of Haining Campus, The First Affiliated Hospital, College of Medicine, Zhejiang University, Haining, China

DOI: https://doi.org/10.3389/fmed.2021.627166
Journal volume & issue: Vol. 8

Abstract

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Chronic disseminated candidiasis (CDC) is a severe complication with high morbidity and mortality in patients with hematological malignancies who have undergone chemotherapy. Blood or sterile liver biopsy cultures are negative due to recurrent empirical antifungal therapy. With the escalating resistance to azole-based antifungal drugs in infection by Candida species, pathogen identification is becoming increasingly important for determining definitive diagnosis and treatment strategy. In this case report, we present, for the first time, diagnostic confirmation of a culture-negative CDC case with Candida tropicalis infection using a combination of metagenomics next-generation sequencing and calcofluor white staining.

Published in Frontiers in Medicine

ISSN: 2296-858X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Medicine (General)
Website: http://www.frontiersin.org/journals/medicine

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