Acute Effects of Aerobic Treadmill Exercise Intensity on Expression of Irisin and FNDC5 in Male Mouse

Abstract

PURPOSE The purpose of this study was to compare the differences in the expression of FNDC5 and irisin according to the intensity of exercise and to determine the most effective exercise intensity for the expression of FNDC5 and irisin immediately after acute exercise. METHODS Studies were conducted using healthy male wild-type C57BLKS/J-m+/m+ mice aged 13-15 weeks. Each group of the mice was randomly assigned to four different groups: control, low intensity (10 m/min, inclination 0°, 25 minutes), moderate intensity (15 m/min, inclination 0°, 25 minutes), high intensity (20 m/min, inclination 0°, 25 minutes). After each exercise, we examined the acute response of irisin and signaling proteins related to irisin. RESULTS The results showed that mRNA expression of FNDC5 tended to increase with increasing exercise intensity, and there was a significant difference between the control group and the moderate intensity exercise group. However, there was no significant difference in protein expression of FNDC5 and irisin in all groups. CONCLUSIONS In conclusion, the acute aerobic exercise did not increase protein expression of FNDC5 and irisin even though aerobic exercise increased the mRNA expression of FNDC5. This results indicated that the 30-minute acute aerobic exercise was not enough stimulation to allow transfer from mRNA expression of FNDC5 to protein expression of FNDC5 and irisin.

Keywords

• peroxisome proliferator-activated receptor-γ coactivator-1α (pgc1α)
• fibronectin typeiii domain-containing protein 5 (fndc5)
• uncoupling protein 1 (ucp1)
• irisin
• aerobic exercise