Detection Of clbB and clbN Genes in Isolated Klebsiella Pneumoniae of Biopsies from Colorectal Cancer Patient in Erbil Province

Abstract

Recently, it has been proved that some of Enterobacteriaceae like Klebsiella pneumoniae, which carry polyketide synthase (PKS) islands, damage human dsDNA by encoding Colibactin genotoxin; and finally, they induce some apoptosis in damaged mucosal cells. This study aimed to isolate the clbN and clbB genes, which are the markers of the PKS genomic island, from PKS cluster in these bacteria. In this study, 100 biopsies were obtained, 50 from them from colorectal cancer patients and another 50 from normal patients as control from PAR International Hospital. Then, all samples were cultured on MacConkey agar medium and blood agar medium for bacterial isolation then, Klebsiella pneumoniae was identified by some biochemical testes (indole test, Cimon citrate test, urease test) and Vitek technique. Finally, The DNA of bacteria was extracted and then were amplified by PCR with specific clbB and clbN gene primers. We obtained (8) isolates out of 50 biopsies from patients with colorectal cancer; and 2 isolates Klebsiella pneumoniae out of 50 non-colorectal cancer patients as control. Further, all (8) klebsiella pneumoniae that we obtained from colorectal cancer patients had both clbB and clbN genes. Whereas 2 klebsiella pneumoniae that obtained from non-colorectal patients had just clbB and they did not have clbN. In this investigation conducted in Erbil province, klebsiella pneumoniae with pks+ island was considerably greater in CRC patients compared to non-cancer individuals.

Keywords

• colorectal cancer
• klebsiella pneumoniae
• pks+ island
• clbb
• clbn
• pcr