Genotypes of carboxypeptidase A1 and gamma‐glutamyltransferase 1 may be useful tools for the diagnosis and the predictor of worrisome features of intraductal papillary mucinous neoplasm in Japan
Shuhei Agawa,
Seiji Futagami,
Ken Nakamura,
Mayu Habiro,
Rie Kawawa,
Yuto Shinagawa,
Rina Motomiya,
Kumiko Kirita,
Teppei Akimoto,
Takeshi Onda,
Tomohide Tanabe,
Nobue Ueki,
Kazufumi Honda,
Kok‐Ann Gwee,
Katsuhiko Iwakiri
Affiliations
Shuhei Agawa
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Seiji Futagami
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Ken Nakamura
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Mayu Habiro
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Rie Kawawa
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Yuto Shinagawa
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Rina Motomiya
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Kumiko Kirita
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Teppei Akimoto
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Takeshi Onda
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Tomohide Tanabe
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Nobue Ueki
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Kazufumi Honda
Department of Bioregulation Graduate School of Medicine, Nippon Medical School Tokyo Japan
Kok‐Ann Gwee
Department of Medicine, Yong Loo Lin School of Medicine National University of Singapore Singapore Singapore
Katsuhiko Iwakiri
Division of Gastroenterology, Department of Internal Medicine Nippon Medical School Tokyo Japan
Abstract Background and Aim This study aimed to clarify whether several single‐nucleotide polymorphisms (SNPs)‐related chronic pancreatitis such as carboxypeptidase A1 (CPA1), carboxypeptidase B1 (CPB1), Gamma‐glutamyltransferase 1 (GGT1), G‐protein‐coupled receptor Class C Group 6 Member A (GPRC6A), and serine protease inhibitor, Kazal type 1 (SPINK‐1) genotypes were associated with clinical characteristics of patients with intraductal papillary mucinous neoplasm (IPMN) and worrisome features of IPMN. Methods We enrolled 100 patients with IPMN and 116 patients as a control. Serum p‐amylase, lipase, trypsin, phospholipase A2 (PLA2), and elastase‐1 levels were measured. An Olympus EUS (GF‐UCT 260) was used to perform endosonography in 100 patients with IPMN. Total EUS score was evaluated using endosonography. DNA was isolated from the duodenal tissue using a commercial system and polymerase chain reaction (PCR) was performed on 7500 Fast PCR System. Results There were no associations between glucose tolerances, lipid levels and genotypes of CPA1, GGT1, GPRC6A, and SPINK‐1 in patients with IPMN. CPA1 genotype was significantly associated with the pathophysiology of IPMN. Then, GGT1 genotype was also significantly associated with EUS total score and the size of cyst more than 20 mm and more than 30 mm as one of worrisome features of IPMN. Conclusion Genotypes of carboxypeptidase A1 and gamma‐glutamyltransferase 1 may be useful tools for the diagnosis and the predictor of worrisome features of IPMN.
