Leptin gene polymorphism Rs7799039; G2548A, metabolic and oxidative stress markers in polycystic ovarian syndrome

Abstract

Objectives: Stress which includes various similar categories such as emotional, inflammatory, oxidative, and metabolic stress is an important component of the Polycystic Ovarian Syndrome (PCOS). The current study examines the relationship of leptin gene polymorphism with metabolic, oxidative stress, and inflammatory markers in polycystic ovarian syndrome. Methods: A total of 300 subjects were enlisted (Control-150; PCOS-150). Commercially available kits were used to measure the glycemic, inflammatory, and lipid profile status. Standard methods were used to examine oxidative stress markers. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for SNP rs7799039 genotyping. Statistical Package for Social Sciences (SPSS) software (version 20.0, IBM, Armonk, NY, USA) was used for statistical analyses. Results: The groups exhibited a difference with statistical significance in terms of body mass index (BMI), insulin, HOMA-IR, Triglyceride, Follicle-stimulating hormone (FSH), Testosterone, vitamin D, leptin, TNF-alpha, IL-4, IL-10, total antioxidant capacity, and myeloperoxidase. Statistically significant differences between HOMA-IR, TC, TG, and inflammatory markers (TNF-alpha, IL-2) were exhibited by the three genotype models. Conclusion: The subjects and controls in this investigation have significantly different rs7799039 genotype distributions. To acquire a better knowledge of the leptin gene’s role in polycystic ovarian syndrome, more research on a larger population of PCOS patients is required.

Keywords

• Leptin gene polymorphism (rs7799039-g2548a)
• Metabolic markers
• Oxidative stress markers
• Polycystic ovarian syndrome