Resequencing of global Lotus corniculatus accessions reveals population distribution and genetic loci, associated with cyanogenic glycosides accumulation and growth traits
Cheng Chen,
Kaixuan Zhang,
Fu Liu,
Xia Wang,
Yang Yao,
Xiaolei Niu,
Yuqi He,
Jun Hong,
Fang Liu,
Qiu Gao,
Yi Zhang,
Yurong Li,
Meijuan Wang,
Jizhen Lin,
Yu Fan,
Kui Ren,
Lunhao Shen,
Bin Gao,
Xue Ren,
Weifei Yang,
Milen I. Georgiev,
Xinquan Zhang,
Meiliang Zhou
Affiliations
Cheng Chen
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Kaixuan Zhang
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Fu Liu
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Xia Wang
Annoroad Gene Technology (Beijing) Co., Ltd.
Yang Yao
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Xiaolei Niu
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource, College of Tropical Crops, Hainan University
Yuqi He
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Jun Hong
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Fang Liu
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Qiu Gao
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Yi Zhang
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Yurong Li
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Meijuan Wang
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Jizhen Lin
National Herbage Gempiasm Bank of China, National Animal Husbandry Service
Yu Fan
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Kui Ren
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Lunhao Shen
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Bin Gao
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Xue Ren
College of Grassland Science and Technology, Sichuan Agricultural University
Weifei Yang
College of Grassland Science and Technology, Sichuan Agricultural University
Milen I. Georgiev
Laboratory of Metabolomics, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences
Xinquan Zhang
College of Grassland Science and Technology, Sichuan Agricultural University
Meiliang Zhou
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
Abstract Background Lotus corniculatus is a widely distributed perennial legume whose great adaptability to different environments and resistance to barrenness make it an excellent forage and ecological restoration plant. However, its molecular genetics and genomic relationships among populations are yet to be uncovered. Result Here we report on a genomic variation map from worldwide 272 L. corniculatus accessions by genome resequencing. Our analysis suggests that L. corniculatus accessions have high genetic diversity and could be further divided into three subgroups, with the genetic diversity centers were located in Transcaucasia. Several candidate genes and SNP site associated with CNglcs content and growth traits were identified by genome-wide associated study (GWAS). A non-synonymous in LjMTR was responsible for the decreased expression of CNglcs synthesis genes and LjZCD was verified to positively regulate CNglcs synthesis gene CYP79D3. The LjZCB and an SNP in LjZCA promoter were confirmed to be involved in plant growth. Conclusion This study provided a large number of genomic resources and described genetic relationship and population structure among different accessions. Moreover, we attempt to provide insights into the molecular studies and breeding of CNglcs and growth traits in L. corniculatus.
