Investigation of The Effect of Radiosensitization of Tungsten Oxide Nanoparticles on AGS Cell Line of Human Stomach Cancer in Megavoltage Photons Radiation

Abstract

Radiotherapy which consists of external beam radiotherapy (EBRT) and internal radioisotope therapy (RIT), has a wide usage for treating cancer as clinical trials.This study provides some conditions to prove that tungsten oxide nanoparticles (WO3) is a radiosensitizer.The peripheral blood mononuclear cells (PBMCs) were used to calculate inhibitory concentration (IC).The AGS cell line exposed the concentration of 89.6 µg/mL (IC20) nanoparticle WO3 that was optimal and its radiosensitization was examined in megavoltage photons radiation of 6 MV x-rays. The sensitivity enhancement ratio (SER) and dose enhancement factor (DEF) was determined 1.24 and 1.68 respectively. We described the mechanisms of creating nanoparticles WO3 toxicity and genotoxicity in different concentrations on AGS cell line. The mean size of WO3 NPs by transmission electron microscopy wasmeasured 31.89 3.82 nm.Tungsten oxide Nanoparticles cause to reduce cell viability, remove membrane and damage to DNA. There was a meaningful increasing in damages to DNA and proliferation cell potency and also significantly reducing cell viability in concentrations more than 100 µg/mL.

Keywords

• radiationtherapy
• radiosensitization
• tungsten oxide nanoparticles
• ags cell line
• pbmc cells