Oral Grapeseed Oil and Sesame Oil in Experimental Acetic Acid-Induced Ulcerative Colitis in Rat

Abstract

Background: Ulcerative colitis (UC) is a multi-factorial disease with unknown etiology and has many clinical manifestations.Objectives: The current study aimed to evaluate the effects of sesame oil (SO) and grapeseed oil (GSO) on acetic acid-induced UC inrats.Materials and Methods: Eighty male rats were divided into eight groups as health control (HC1), received normal saline; HC2, receivedSO; HC3, received GSO; negative control (NC), UC and normal saline; positive control (PC), UC and mesalamine; SO, UC andSO; GSO, UC and GSO, and SO + GSO. The daily weight changes, serum levels of oxidative stress markers and lipid profile plus colonmacroscopic and microscopic histological changes were measured at the end of the seventh day.Results: Significant differences were detected betweenHC1 and PC on the 3rd (P = 0.002), 4th (0.013) and 6th days (0.014) and betweenHC1 and NC on the 4th day (0.027) in weight of rats. Use of GSO alone or in combination with SO decreased the extent of the changesboth in macroscopic and microscopic indices and also at the inflammation level. The most significant decrease in the MDA leveland the most obvious increase in the TAC belonged to the GSO group in comparison to the NC group. The lowest cholesterol (51.43± 5.62 mg/dL) and HDL levels (29.29 ± 6.24 mg/dL) were detected in response to SO consumption in comparison to NC group (P =0.030 and P = 0.257, respectively).Conclusions: GSO in combination with SO may be considered as the treatment of choice for UC based on antioxidant andhistopathological evaluations.

Keywords

• grapeseed oil
• sesame oil
• ulcerative colitis
• oxidative stress
• histopathology