Comparative characteristics of respiration and oxidative phosphorylation in mitochondria of cells of mouse liver and lymphoma NK/Ly

Abstract

Parameters of respiration and oxidative phosphorylation in mouse liver cell mitochondria and experimental lymphoma NK/Ly was investigated. The suspensions of mitochondria were obtained by the differential centrifugation. Oxygen uptake rate was measured using platinum polarographic Clark electrode. α-Ketoglutarate and succinate were used as substrates. It was established that the rate of oxygen uptake in mitochondria of mouse liver cells is higher in all investigated metabolic states compared to the rate of oxygen uptake in mitochondria of lymphoma NK/Ly cells when both α-ketoglu­tarate and succinate were used as substrates. Also we found lower rates of respiratory control and longer time of phosphorylation in tumor cell mitochondria compared to liver cell mitochondria for the oxidation of α-ketoglutarate, while the ratio of ADP/O was lower in mitochondria of lymphoma cells for the oxidation of succinate.

Keywords

• mitochondria
• respiration
• oxidative phosphorylation
• liver mouse
• lymphoma Nemeth-Kellner