Strongyloides stercoralis infection reduces Fusicatenibacter and Anaerostipes in the gut and increases bacterial amino-acid metabolism in early-stage chronic kidney disease
Na T.D. Tran,
Apisit Chaidee,
Achirawit Surapinit,
Manachai Yingklang,
Sitiruk Roytrakul,
Sawanya Charoenlappanit,
Porntip Pinlaor,
Nuttanan Hongsrichan,
Hai Nguyen Thi,
Sirirat Anutrakulchai,
Ubon Cha'on,
Somchai Pinlaor
Affiliations
Na T.D. Tran
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medical Laboratory Science, Danang University of Medical Technology and Pharmacy, Danang, Viet Nam; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Apisit Chaidee
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Achirawit Surapinit
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
Manachai Yingklang
Faculty of Public Health, Burapha University, Chonburi, Thailand
Sitiruk Roytrakul
Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand
Sawanya Charoenlappanit
Functional Proteomics Technology Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand
Porntip Pinlaor
Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Nuttanan Hongsrichan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Hai Nguyen Thi
Department of Parasitology, Faculty of Basic Medicine, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen, Viet Nam
Sirirat Anutrakulchai
Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Ubon Cha'on
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand
Somchai Pinlaor
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Chronic Kidney Disease Prevention in Northeastern Thailand, Khon Kaen University, Khon Kaen, Thailand; Corresponding author. Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Understanding gut bacterial composition and proteome changes in patients with early-stage chronic kidney disease (CKD) could lead to better methods of controlling the disease progression. Here, we investigated the gut microbiome and microbial functions in patients with S. stercoralis infection (strongyloidiasis) and early-stage CKD. Thirty-five patients with early stages (1–3) of CKD were placed in two groups matched for population characteristics and biochemical parameters, 12 patients with strongyloidiasis in one group and 23 uninfected patients in the other. From every individual, a sample of their feces was obtained and processed for 16S rRNA sequencing and metaproteomic analysis using tandem liquid chromatography-mass spectrometry (LC-MS/MS). Strongyloides stercoralis infection per se did not significantly alter gut microbial diversity. However, certain genera (Bacteroides, Faecalibacterium, Fusicatenibacter, Sarcina, and Anaerostipes) were significantly more abundant in infection-free CKD patients than in infected individuals. The genera Peptoclostridium and Catenibacterium were enriched in infected patients. Among the significantly altered genera, Fusicatenibacter and Anaerostipes were the most correlated with renal parameters. The relative abundance of members of the genus Fusicatenibacter was moderately positively correlated with estimated glomerular filtration rate (eGFR) (r = 0.335, p = 0.049) and negatively with serum creatinine (r = −0.35, p = 0.039). Anaerostipes, on the other hand, showed a near-significant positive correlation with eGFR (r = 0.296, p = 0.084). Individuals with S. stercoralis infection had higher levels of bacterial proteins involved in amino-acid metabolism. Analysis using STITCH predicted that bacterial amino-acid metabolism may also be involved in the production of colon-derived uremic toxin (indole), a toxic substance known to promote CKD. Strongyloides stercoralis infection is, therefore, associated with reduced abundance of Fusicatenibacter and Anaerostipes (two genera possibly beneficial for kidney function) and with increased bacterial amino-acid metabolism in the early-stages of CKD, potentially producing uremic toxin. This study provides useful information for prevention of progression of CKD beyond the early stages.
