Zhongguo shuxue zazhi (Jun 2024)
Effect of radiation grafting modification of PBT on platelet function
Abstract
Objective To investigate the effects of polybutylene terephthalate(PBT) grafted with various monomers, such as acrylic acid(AA), acrylamide(AM), sodium styrene sulfonate(SSS), AA+ AM and AA+ SSS on platelet adhesion and function. Methods The AA, AM, SSS, AA+ AM, and AA+ SSS were grafted onto the surface of PBT by γ-ray irradiation, and the grafted PBT was characterized by fourier transform infrared spectrometry(FTIR) and wetting time. Platelet activation and aggregation of different monomers grafted with PBT were observed by scanning electron microscopy(SEM). Platelet concentration, maximum aggregation ability, positive expression rate of CD62p and hypotonic shock rate(HSR)of PBT grafted with different monomers were tested to study their effect on platelet adhesion and function. Results The characteristic absorption peaks of AA, AM and SSS appeared in FTIR, and the hydrophilicity of the grafted material was improved obviously, indicating that the monomer was grafted successfully. The results of SEM showed that the degree of platelet activation and aggregation caused by the original PBT was significantly higher than that of modified ones. Compared with the original platelets, the platelet concentration of PBT was(533.00±4.58 vs 672.00±3.61)×109/L, the maximum platelet aggregation rate was(48.80±0.96 vs 58.60±1.37)%, the positive expression rate of CD62p was(45.35±0.58 vs 39.90±0.52)%, and the platelet HSR was(48.74±0.46 vs 51.86±0.93)%(P<0.05). Compared with the original PBT, the platelet loss and platelet function damage caused by PBT grafted with different monomers decreased significantly(P<0.05). PBT-(AA+ AM) had the least comprehensive effect on platelets [platelet concentration(637.00±2.65)×109/L, maximum platelet aggregation rate(62.45±0.61)%, positive expression rate of CD62p(37.39±0.42)%, platelet HSR(53.51±0.58)%]. Conclusion The comprehensive effect of PBT grafted with AA+ AM on platelet adhesion and function is significantly lower than that of PBT grafted with other monomers, so it is anticipated to apply filtering and removing leukocytes in platelet preparations.
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