Frontiers in Immunology (Mar 2016)

Distinct mechanisms regulate Lck spatial organization in activated T cells

  • Natasha eKapoor-Kaushik,
  • Elizabeth eHinde,
  • Elizabeth eHinde,
  • Ewoud Bernardus Compeer,
  • Yui eYamamoto,
  • Yui eYamamoto,
  • Felix eKraus,
  • Zhengmin eYang,
  • Zhengmin eYang,
  • Jieqiong eLou,
  • Jieqiong eLou,
  • Thibault eTabarin,
  • Thibault eTabarin,
  • Sophie Victoria Pageon,
  • Sophie Victoria Pageon,
  • Katharina eGaus,
  • Katharina eGaus,
  • Jeremie eRossy,
  • Jeremie eRossy

DOI
https://doi.org/10.3389/fimmu.2016.00083
Journal volume & issue
Vol. 7

Abstract

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Phosphorylation of the T cell receptor (TCR) by the kinase Lck is the first detectable signaling event upon antigen engagement. The distribution of Lck within the plasma membrane, its conformational state, kinase activity and protein interactions all contribute to determine how efficiently Lck phosphorylates the engaged TCR. Here we used cross-correlation raster image spectroscopy (ccRICS) and photoactivated localization microscopy (PALM) to identify two mechanisms of Lck clustering: an intrinsic mechanism of Lck clustering induced by locking Lck in its open conformation, and an extrinsic mechanism of clustering controlled by the phosphorylation of tyrosine 192, which regulates the affinity of Lck SH2 domain. Both mechanisms of clustering were differently affected by the absence of the kinase Zap70 or the adaptor Lat. We further observed that the adaptor TSAd bound to and promoted the diffusion of Lck when it is phosphorylated on tyrosine 192. Our data suggest that while Lck open conformation drives aggregation and clustering, the spatial organization of Lck is further controlled by signaling events downstream of TCR phosphorylation.

Keywords