The eff ect of two Iranian viper snake; vipera albicornuta (zanjani) and vipera latifi i (lattifi i) venoms on the viability of rat bone marrow mesenchymal stem cells in vitro and in vivo

Abstract

Snake venom is a complex mixture of different compounds which have potential pharmacological properties and may affect mesenchymal stem cells (MSCs). In this study we investigate the effects of two Iranian vipers; vipera albicornuta and vipera latifii crude venoms on the viability of MScs in vivo and in vitro. The cells in in vitro tests were treated with different concentrations (1, 2, 3, 4 and 5 μg/100μl) of mentioned venoms for 24, 48 and 72 hours.The cells in in vivo experiment only were treated with v. Latifii venom at the concentration of 1μg/100μl and time intervals as in vitro tests. The cell viability in in vitro experiment was assessed using MTT assay. The results of in vitro experiments showed that maximum cell viability was observed at concentrations of 1 and 2 μg/100μl of v. albicornuta and v. latifi ivenoms aft er 48 and 72 hours, respectively. The results of in vivo experiment showed that the cells treated with v. Latifii venom for 72 hours in situ have the highest proliferation rate after passages three, four and five in comparison to control. The results of this study showed that the v. albicornuta and v. latifi i venoms can affect the confluence and viability of the MSCs.

Keywords

• mesenchymal stem cells
• venom
• viper snake
• cell culture
• bone marrow