Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells

Haote Han; Yanhui Yang; Zhipan Wu; Bing Liu; Lijun Dong; Hongwei Deng; Jingkui Tian; Hetian Lei

Biomedicine & Pharmacotherapy (Jan 2021)

Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells

Haote Han,
Yanhui Yang,
Zhipan Wu,
Bing Liu,
Lijun Dong,
Hongwei Deng,
Jingkui Tian,
Hetian Lei

Affiliations

Haote Han: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, PR China; Zhejiang-Malaysia Joint Research Center for Traditional Medicine, Zhejiang University, Hangzhou, 310027, PR China; Schepens Eye Research Institute of Massachusetts Eye and Ear, Boston, MA, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA, USA
Yanhui Yang: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, PR China
Zhipan Wu: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, PR China; Zhejiang-Malaysia Joint Research Center for Traditional Medicine, Zhejiang University, Hangzhou, 310027, PR China
Bing Liu: Schepens Eye Research Institute of Massachusetts Eye and Ear, Boston, MA, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA, USA; Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510005, Guangzhou, PR China
Lijun Dong: Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, Shenzhen, Guangdong Province, PR China
Hongwei Deng: Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, Shenzhen, Guangdong Province, PR China; Corresponding authors at: 18 Zetian Road, Shenzhen Eye Hospital, Futian District, Shenzhen, Guangdong Province, PR China.
Jingkui Tian: College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, PR China; Zhejiang-Malaysia Joint Research Center for Traditional Medicine, Zhejiang University, Hangzhou, 310027, PR China; Corresponding author at: 148 Tianmushan Road, Zhejiang University, Hangzhou, Zhejiang province, PR China.
Hetian Lei: Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, Shenzhen, Guangdong Province, PR China; Corresponding authors at: 18 Zetian Road, Shenzhen Eye Hospital, Futian District, Shenzhen, Guangdong Province, PR China.

Journal volume & issue: Vol. 133
p. 110999

Abstract

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Abnormal angiogenesis is associated with intraocular diseases such as proliferative diabetic retinopathy and neovascular age-related macular degeneration, and current therapies for these eye diseases are not satisfactory. The purpose of this study was to determine whether capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, can inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis signaling events and cellular responses in primary human retinal microvascular endothelial cells (HRECs). Our study revealed that the capilliposide B IC50 for HRECs was 8.5 μM at 72 h and that 1 μM capilliposide B specifically inhibited VEGF-induced activation of VEGFR2 and its downstream signaling enzymes Akt and Erk. In addition, we discovered that this chemical effectively blocked VEGF-stimulated proliferation, migration and tube formation of the HRECs, suggesting that capilliposide B is a promising prophylactic for angiogenesis-associated diseases such as proliferative diabetic retinopathy.

Published in Biomedicine & Pharmacotherapy

ISSN: 0753-3322 (Print); 1950-6007 (Online)
Publisher: Elsevier
Country of publisher: France
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.journals.elsevier.com/biomedicine-and-pharmacotherapy

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