Growth profile and partial characterization of bacteriocin produced by Lactobacillus plantarum ATM11 isolated from slaughterhouse soil

Abstract

In this study, we report on the growth profile and partial characterization of a bacteriocin produced by Lactobacillus plantarum ATM11 isolated from goat slaughterhouse soil. Among eight bacteriocin-producing bacteria from 25 Lactobacilli isolates of goat slaughterhouse soil, the higher-yielding bacteria were selected and identified as Lactobacillus plantarum based on the morphology, biochemical characteristics, and partial 16S rRNA gene sequence. The physico-chemical condition effects of temperature, pH, enzymes, and detergents of bacteriocin activity were studied. The optimum temperature for bacteriocin activity was found to be 60 C. The optimum pH for bacteriocin activity was found to be in the range of 5.0-6.0. Detergents such as sodium dodecyl sulphate (SDS), Triton X-100, and ethylene diamine tetra-acetic acid (EDTA) were influenced the bacteriocin activity, whereas urea inhibited the activity. Asparaginase and -amylase enzymes influenced the activity, whereas protease, proteinase K, and lysozyme inhibited bacteriocin activity. The present study revealed that the action mode of the produced bacteriocin has a bacteriostatic effect against Bacillus cereus MTCC 1272 and Micrococcus leuteus MTCC 2987. Thus, it can be used as a bio-preservative agent for different foods in the future.

Keywords

• bacteriocin
• biochemical tests
• 16S rRNA sequencing
• L. plantarum ATM11
• growth profile
• physicochemical characterization