Neural Regeneration Research (Jan 2015)
Human amniotic epithelial cell transplantation for the repair of injured brachial plexus nerve: evaluation of nerve viscoelastic properties
- Hua Jin,
- Qi Yang,
- Feng Ji,
- Ya-jie Zhang,
- Yan Zhao,
- Min Luo
Affiliations
- Hua Jin
- Qi Yang
- Feng Ji
- Ya-jie Zhang
- Yan Zhao
- Min Luo
- DOI
- https://doi.org/10.4103/1673-5374.152380
- Journal volume & issue
-
Vol. 10,
no. 2
pp. 260 – 265
Abstract
The transplantation of embryonic stem cells can effectively improve the creeping strength of nerves near an injury site in animals. Amniotic epithelial cells have similar biological properties as embryonic stem cells; therefore, we hypothesized that transplantation of amniotic epithelial cells can repair peripheral nerve injury and recover the creeping strength of the brachial plexus nerve. In the present study, a brachial plexus injury model was established in rabbits using the C 6 root avulsion method. A suspension of human amniotic epithelial cells was repeatedly injected over an area 4.0 mm lateral to the cephal and caudal ends of the C 6 brachial plexus injury site (1 × 10 6 cells/mL, 3 μL/injection, 25 injections) immediately after the injury. The results showed that the decrease in stress and increase in strain at 7,200 seconds in the injured rabbit C 6 brachial plexus nerve were mitigated by the cell transplantation, restoring the viscoelastic stress relaxation and creep properties of the brachial plexus nerve. The forepaw functions were also significantly improved at 26 weeks after injury. These data indicate that transplantation of human amniotic epithelial cells can effectively restore the mechanical properties of the brachial plexus nerve after injury in rabbits and that viscoelasticity may be an important index for the evaluation of brachial plexus injury in animals.
Keywords
- Alzheimer′s disease
- amyloid-β
- astrocytes
- Ca 2+
- calcilytic
- calcium-sensing receptor
- nitromemantine
- NPS 2143
- α7-nicotinic acetylcholine receptor
- nerve regeneration
- spinal cord injury
- surgical decompression
- tumor necrosis factor α
- cell apoptosis
- neurological function
- neural regeneration
- nerve regeneration
- spinal cord injury
- contusion
- Nogo-A
- axon growth
- immunohistochemistry
- fluorescent quantitative PCR
- neural regeneration
- nerve regeneration
- spinal cord injury
- Schwann cells
- cell transplantation
- edaravone
- motor function
- electrophysiological function
- neural regeneration
- nerve regeneration
- electroacupuncture
- intervertebral disc
- blood circulation
- inflammation
- neuroprotection
- motor function
- neurons
- NSFC grants
- neural regeneration
- nerve regeneration
- astrocytoma
- mice
- immunodeficiency (BALB/c) mice
- Notch
- nestin
- glial fibrillary acidic protein
- CD133
- spinal cord
- brain
- MRI
- neural regeneration
- nerve regeneration
- earthquake
- peripheral nerve injury
- LSUHSC score
- compartment syndrome
- surgery therapy
- physiotherapy
- nerve decompression
- neural regeneration
- nerve regeneration
- brachial plexus injury
- human amniotic epithelial cells
- forepaw function
- stress relaxation
- creep
- viscoelasticity
- neural regeneration