Perforated Patch Clamp Recordings in ex vivo Brain Slices from Adult Mice
Simon Hess,
Helmut Wratil,
Peter Kloppenburg
Affiliations
Simon Hess
Department of Biology, Institute for Zoology, Cologne Excellence Cluster in Aging Associated Diseases (CECAD), and Center of Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Helmut Wratil
Department of Biology, Institute for Zoology, Cologne Excellence Cluster in Aging Associated Diseases (CECAD), and Center of Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Peter Kloppenburg
Department of Biology, Institute for Zoology, Cologne Excellence Cluster in Aging Associated Diseases (CECAD), and Center of Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany
Intracellular signaling pathways directly and indirectly regulate neuronal activity. In cellular electrophysiological measurements with sharp electrodes or whole-cell patch clamp recordings, there is a great risk that these signaling pathways are disturbed, significantly altering the electrophysiological properties of the measured neurons. Perforated-patch clamp recordings circumvent this issue, allowing long-term electrophysiological recordings with minimized impairment of the intracellular milieu. Based on previous studies, we describe a superstition-free protocol that can be used to routinely perform perforated patch clamp recordings for current and voltage measurements.
