Сибирский научный медицинский журнал (Aug 2024)
Effect of production factors on the relative telomere length of ICR mice
Abstract
Currently, the issues of studying the quantitative assessment of the isolated effects of physical and chemical occupational factors of low intensity, severity and intensity of the labor process on the telomere relative length (TRL) are relevant in order to establish the mechanisms of development of premature aging. The aim is to evaluate the effect of isolated exposure to low–intensity physical and chemical occupational factors on the relative length of telomeres in an animal experiment. Material and methods. ICR mice (n = 65) are distributed in equal numbers into 3 test groups (exposure to vibration, noise, chemical exposure) and 1 control group (contained in comfortable conditions and not exposed to these factors). The duration of the experiment was 90 days, samples for DNA isolation from the striated muscle tissue of ICR mice thigh were taken on days 0, 30, 60 and 90. TRL was measured using real time PCR. Results and discussion. The results of the study indicate a significant shortening of TRL on the 90th day in the control group compared to the initial indicators, which may indicate the general processes of aging of animals. A long (90-day) stay of ICR mice in conditions simulating the isolated influence of various occupational hazard factors (vibration, noise, chemicals) operating at a level of 1.5 MAC, MPL, was accompanied by unidirectional dynamics of TRL. The chemical factor has the greatest impact on TDC (a decrease in TDT relative to the value of the control group is noted on the 30th, 60th and 90th days of the experiment), the physical factor causes a shortening of TDT after 60 and 90 days. Conclusions. Further study of the isolated influence of occupational factors on the TRL change in model organisms will contribute to the establishment of mechanisms that prevent the development of pathological processes in workers under conditions of production hazards.
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