The Effects of Incremental Doses of Aflatoxin B<sub>1</sub> on In Vitro Ruminal Nutrient Digestibility and Fermentation Profile of a Lactating Dairy Cow Diet in a Dual-Flow Continuous Culture System
Felipe Xavier Amaro,
Yun Jiang,
Kathy Arriola,
Matheus R. Pupo,
Bruna C. Agustinho,
Sarah L. Bennett,
James R. Vinyard,
Lais Tomaz,
Richard R. Lobo,
Andres Pech-Cervantes,
Jose A. Arce-Cordero,
Antonio P. Faciola,
Adegbola Tolulope Adesogan,
Diwakar Vyas
Affiliations
Felipe Xavier Amaro
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Yun Jiang
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Kathy Arriola
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Matheus R. Pupo
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Bruna C. Agustinho
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Sarah L. Bennett
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
James R. Vinyard
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Lais Tomaz
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Richard R. Lobo
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Andres Pech-Cervantes
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Jose A. Arce-Cordero
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Antonio P. Faciola
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Adegbola Tolulope Adesogan
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Diwakar Vyas
Department of Animal Sciences, University of Florida, Gainesville, FL 32611, USA
Aflatoxin B1 (AFB1) is a mycotoxin known to impair human and animal health. It is also believed to have a deleterious effect on ruminal nutrient digestibility under in vitro batch culture systems. The objective of this study was to evaluate the effects of increasing the dose of AFB1 on ruminal dry matter and nutrient digestibility, fermentation profile, and N flows using a dual-flow continuous culture system fed a diet formulated for lactating dairy cows. Eight fermenter vessels were used in a replicated 4 × 4 Latin square design with 10 d periods (7 d adaptation and 3 d sample collection). Treatments were randomly applied to fermenters on diet DM basis: (1) 0 μg of AFB1/kg of DM (Control); (2) 50 μg of AFB1/kg of DM (AF50); (3) 100 μg of AFB1/kg of DM (AF100); and (4) 150 μg of AFB1/kg of DM (AF150). Treatments did not affect nutrient digestibility, fermentation, and N flows. Aflatoxin B1 concentration in ruminal fluid increased with dose but decreased to undetectable levels after 4 h post-dosing. In conclusion, adding incremental doses of AFB1 did not affect ruminal fermentation, digestibility of nutrients, and N flows in a dual-flow continuous culture system fed diets formulated for lactating dairy cows.
