Minimal residual disease detected by droplet digital PCR in peripheral blood stem cell grafts has a prognostic impact on high-risk neuroblastoma patients
Nanako Nino,
Toshiaki Ishida,
Naoko Nakatani,
Kyaw San Lin,
Kaung Htet Nay Win,
Cho Yee Mon,
Akihiro Nishimura,
Shotaro Inoue,
Akihiro Tamura,
Nobuyuki Yamamoto,
Suguru Uemura,
Atsuro Saito,
Takeshi Mori,
Daiichiro Hasegawa,
Yoshiyuki Kosaka,
Kandai Nozu,
Noriyuki Nishimura
Affiliations
Nanako Nino
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Toshiaki Ishida
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Naoko Nakatani
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Kyaw San Lin
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Kaung Htet Nay Win
Department of Public Health, Kobe University Graduate School of Health Science, Kobe, Japan
Cho Yee Mon
Department of Public Health, Kobe University Graduate School of Health Science, Kobe, Japan
Akihiro Nishimura
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Shotaro Inoue
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Akihiro Tamura
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Nobuyuki Yamamoto
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Suguru Uemura
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Atsuro Saito
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Takeshi Mori
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Daiichiro Hasegawa
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Yoshiyuki Kosaka
Department of Hematology and Oncology, Kobe Children's Hospital, Kobe, Japan
Kandai Nozu
Department of Pediatrics, Kobe University Graduate School of Medicine, Kobe, Japan
Noriyuki Nishimura
Department of Public Health, Kobe University Graduate School of Health Science, Kobe, Japan; Corresponding author.
Abstracts: More than half of high-risk neuroblastoma (NB) patients have experienced relapse due to the activation of chemoresistant minimal residual disease (MRD) even though they are treated by high-dose chemotherapy with autologous peripheral blood stem cell (PBSC) transplantation. Although MRD in high-risk NB patients can be evaluated by quantitative PCR with several sets of neuroblastoma-associated mRNAs (NB-mRNAs), the prognostic significance of MRD in PBSC grafts (PBSC-MRD) is unclear. In the present study, we collected 20 PBSC grafts from 20 high-risk NB patients and evaluated PBSC-MRD detected by droplet digital PCR (ddPCR) with 7NB-mRNAs (CRMP1, DBH, DDC, GAP43, ISL1, PHOX2B, and TH mRNA). PBSC-MRD in 11 relapsed patients was significantly higher than that in 9 non-relapsed patients. Patients with a higher PBSC-MRD had a lower 3-year event-free survival (P = 0.0148). The present study suggests that PBSC-MRD detected by ddPCR with 7NB-mRNAs has a prognostic impact on high-risk NB patients.
