Impact of Ultrasound- and Microwave-Assisted Extraction on Bioactive Compounds and Biological Activities of <i>Jania rubens</i> and <i>Sargassum muticum</i>
Kahina Hamamouche,
Zoubida Elhadj,
Latifa Khattabi,
Wafa Zahnit,
Brahim Djemoui,
Omar Kharoubi,
Walid Boussebaa,
Mouhamed Bouderballa,
Mohammed EL Moustapha Kallouche,
Sabry M. Attia,
Sheikh F. Ahmad,
Maria Atanassova,
Mohammed Messaoudi
Affiliations
Kahina Hamamouche
Environmental Monitoring Network Laboratory, Department of Biology, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, Algeria
Zoubida Elhadj
Laboratory for Environmental and Materials Sciences Studies, Ahmed Ben Bella University, Oran 31000, Algeria
Latifa Khattabi
Biotechnology Research Center-C.R.B.T, Constantine 25017, Algeria
Wafa Zahnit
Department of Chemistry, Faculty of Sciences, University of Ferhat ABBAS Setif 1, El Bez 19000, Algeria
Brahim Djemoui
Laboratory of Excellence in Applied Organic Synthesis, Faculty of Exact and Applied Sciences, Ahmed Ben Bella University, Oran 31000, Algeria
Omar Kharoubi
Laboratory of Experimental Biotoxicology, Biodepollution and Phytoremediation, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, Algeria
Walid Boussebaa
Scientific and Technical Research Center in Physico-Chemical Analysis CRAPC BP384, Bou-Ismail 42004, Algeria
Mouhamed Bouderballa
Environmental Monitoring Network Laboratory, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, Algeria
Mohammed EL Moustapha Kallouche
Environmental Monitoring Network Laboratory, Faculty of Natural and Life Sciences, Ahmed Ben Bella University, Oran 31000, Algeria
Sabry M. Attia
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Sheikh F. Ahmad
Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
Maria Atanassova
Scientific Consulting, Chemical Engineering, University of Chemical Technology and Metallurgy, 1734 Sofia, Bulgaria
Mohammed Messaoudi
Nuclear Research Centre of Birine, Ain Oussera 17200, Algeria
This study represents the first investigation into the ultrasonic and microwave extraction of bioactive metabolites from Jania rubens (J. rubens) (red seaweed) and Sargassum. muticum (S. muticum) (brown seaweed), with a focus on their biological activities. The research compares ultrasound-assisted extraction (UAE) with microwave-assisted extraction (MAE) utilizing a hydromethanolic solvent to evaluate their effects on these seaweeds’ bioactive compounds and biological activities. The assessment included a series of antioxidant essays: DPPH, ABTS, phenanthroline, and total antioxidant capacity, followed by enzyme inhibition activities: alpha-amylase and urease. Results revealed significant proportions of phenolic compounds, ranging from 48.31 ± 0.32 to 74.42 ± 0.80 μg GAE/mg, depending on the extraction method. The extracts demonstrated a high antioxidant activity, with IC50 values ranging from 26.58 ± 0.39 to 87.55 ± 0.69 μg/mL. Notably, the MAE extract of S. muticum showed a value of 48.11 ± 2.75 μg/mL for alpha-amylase inhibition, which is strictly superior to the reference acarbose with an IC50 equal to 3431.01 μg/mL. UPLC-ESI-MS/MS analysis identified 14 bioactive compounds. The proportion of riboflavin with MAE was 70.58% and 59.11% for J. rubens and S. muticum fractions, respectively. These findings underscore the critical influence of extraction technique selection on bioactive compounds’ yield and efficiency, highlighting the potential of algal biomass as a sustainable alternative in various applications.
