Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

Tam Vu; Alexander Vallmitjana; Joshua Gu; Kieu La; Qi Xu; Jesus Flores; Jan Zimak; Jessica Shiu; Linzi Hosohama; Jie Wu; Christopher Douglas; Marian L. Waterman; Anand Ganesan; Per Niklas Hedde; Enrico Gratton; Weian Zhao

doi:10.1038/s41467-021-27798-0

Nature Communications (Jan 2022)

Spatial transcriptomics using combinatorial fluorescence spectral and lifetime encoding, imaging and analysis

Tam Vu,
Alexander Vallmitjana,
Joshua Gu,
Kieu La,
Qi Xu,
Jesus Flores,
Jan Zimak,
Jessica Shiu,
Linzi Hosohama,
Jie Wu,
Christopher Douglas,
Marian L. Waterman,
Anand Ganesan,
Per Niklas Hedde,
Enrico Gratton,
Weian Zhao

Affiliations

Tam Vu: Department of Biomedical Engineering, University of California, Irvine
Alexander Vallmitjana: Department of Biomedical Engineering, University of California, Irvine
Joshua Gu: Sue and Bill Gross Stem Cell Research Center, University of California, Irvine
Kieu La: Department of Biomedical Engineering, University of California, Irvine
Qi Xu: Department of Biomedical Engineering, University of California, Irvine
Jesus Flores: Sue and Bill Gross Stem Cell Research Center, University of California, Irvine
Jan Zimak: Department of Pharmaceutical Sciences, University of California, Irvine
Jessica Shiu: Department of Dermatology, University of California, Irvine
Linzi Hosohama: Department of Microbiology and Molecular Genetics, University of California, Irvine
Jie Wu: Department of Biological Chemistry, University of California, Irvine
Christopher Douglas: Department of Pathology & Laboratory Medicine, University of California, Irvine
Marian L. Waterman: Department of Microbiology and Molecular Genetics, University of California, Irvine
Anand Ganesan: Department of Biological Chemistry, University of California, Irvine
Per Niklas Hedde: Laboratory for Fluorescence Dynamics, University of California, Irvine
Enrico Gratton: Department of Biomedical Engineering, University of California, Irvine
Weian Zhao: Department of Biomedical Engineering, University of California, Irvine

DOI: https://doi.org/10.1038/s41467-021-27798-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Spatial-omics methods with ease-of-use and high multiplexing are in demand. Here the authors report Multi Omic Single-scan Assay with Integrated Combinatorial Analysis (MOSAICA) which uses Spectral and Fluorescence Lifetime Imaging and Microscopy; they apply this to co-detection of mRNA and protein.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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