Concordant and Heterogeneity of Single-Cell Transcriptome in Cardiac Development of Human and Mouse

Mengyue Shang; Mengyue Shang; Mengyue Shang; Yi Hu; Yi Hu; Yi Hu; Huaming Cao; Qin Lin; Qin Lin; Qin Lin; Na Yi; Na Yi; Na Yi; Junfang Zhang; Yanqiong Gu; Yujie Yang; Siyu He; Siyu He; Siyu He; Min Lu; Luying Peng; Luying Peng; Luying Peng; Luying Peng; Luying Peng; Li Li; Li Li; Li Li; Li Li; Li Li

doi:10.3389/fgene.2022.892766

Frontiers in Genetics (Jun 2022)

Concordant and Heterogeneity of Single-Cell Transcriptome in Cardiac Development of Human and Mouse

Mengyue Shang,
Mengyue Shang,
Mengyue Shang,
Yi Hu,
Yi Hu,
Yi Hu,
Huaming Cao,
Qin Lin,
Qin Lin,
Qin Lin,
Na Yi,
Na Yi,
Na Yi,
Junfang Zhang,
Yanqiong Gu,
Yujie Yang,
Siyu He,
Siyu He,
Siyu He,
Min Lu,
Luying Peng,
Luying Peng,
Luying Peng,
Luying Peng,
Luying Peng,
Li Li,
Li Li,
Li Li,
Li Li,
Li Li

Affiliations

Mengyue Shang: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Mengyue Shang: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Mengyue Shang: Institute of Medical Genetics, Tongji University, Shanghai, China
Yi Hu: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Yi Hu: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Yi Hu: Institute of Medical Genetics, Tongji University, Shanghai, China
Huaming Cao: Department of Cardiology, Shanghai Shibei Hospital, Shanghai, China
Qin Lin: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Qin Lin: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Qin Lin: Institute of Medical Genetics, Tongji University, Shanghai, China
Na Yi: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Na Yi: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Na Yi: Institute of Medical Genetics, Tongji University, Shanghai, China
Junfang Zhang: Institute of Medical Genetics, Tongji University, Shanghai, China
Yanqiong Gu: Institute of Medical Genetics, Tongji University, Shanghai, China
Yujie Yang: Institute of Medical Genetics, Tongji University, Shanghai, China
Siyu He: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Siyu He: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Siyu He: Institute of Medical Genetics, Tongji University, Shanghai, China
Min Lu: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Luying Peng: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Luying Peng: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Luying Peng: Institute of Medical Genetics, Tongji University, Shanghai, China
Luying Peng: Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China
Luying Peng: Research Units of Origin and Regulation of Heart Rhythm, Chinese Academy of Medical Sciences, Beijing, China
Li Li: Key Laboratory of Arrhythmias, Ministry of Education of China, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Li Li: Heart Health Center, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China
Li Li: Institute of Medical Genetics, Tongji University, Shanghai, China
Li Li: Department of Medical Genetics, Tongji University School of Medicine, Shanghai, China
Li Li: Research Units of Origin and Regulation of Heart Rhythm, Chinese Academy of Medical Sciences, Beijing, China

DOI: https://doi.org/10.3389/fgene.2022.892766
Journal volume & issue: Vol. 13

Abstract

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Normal heart development is vital for maintaining its function, and the development process is involved in complex interactions between different cell lineages. How mammalian hearts develop differently is still not fully understood. In this study, we identified several major types of cardiac cells, including cardiomyocytes (CMs), fibroblasts (FBs), endothelial cells (ECs), ECs/FBs, epicardial cells (EPs), and immune cells (macrophage/monocyte cluster, MACs/MONOs), based on single-cell transcriptome data from embryonic hearts of both human and mouse. Then, species-shared and species-specific marker genes were determined in the same cell type between the two species, and the genes with consistent and different expression patterns were also selected by constructing the developmental trajectories. Through a comparison of the development stage similarity of CMs, FBs, and ECs/FBs between humans and mice, it is revealed that CMs at e9.5 and e10.5 of mice are most similar to those of humans at 7 W and 9 W, respectively. Mouse FBs at e10.5, e13.5, and e14.5 are correspondingly more like the same human cells at 6, 7, and 9 W. Moreover, the e9.5-ECs/FBs of mice are most similar to that of humans at 10W. These results provide a resource for understudying cardiac cell types and the crucial markers able to trace developmental trajectories among the species, which is beneficial for finding suitable mouse models to detect human cardiac physiology and related diseases.

Published in Frontiers in Genetics

ISSN: 1664-8021 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Genetics
Website: http://journal.frontiersin.org/journal/genetics

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