Nature Communications (Nov 2021)

Engineering a PAM-flexible SpdCas9 variant as a universal gene repressor

  • Jian Wang,
  • Yuxi Teng,
  • Ruihua Zhang,
  • Yifei Wu,
  • Lei Lou,
  • Yusong Zou,
  • Michelle Li,
  • Zhong-Ru Xie,
  • Yajun Yan

DOI
https://doi.org/10.1038/s41467-021-27290-9
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 10

Abstract

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SpCas9 has a NGG PAM requirement that limits its DNA target range. Here the authors present a PAM-expanded SpdCas9 variant SpdNG-LWQT to target NRN PAMs.