Molecular characterization, phylogenetic and in silico sequence analysis data of trehalose biosynthesis genes; otsA and otsB from the deep sea halophilic actinobacteria, Streptomyces qinglanensis NIOT-DSA03
Balakrishnan Meena,
Lawrance Anburajan,
Nambali Valsalan Vinithkumar,
Ramalingam Kirubagaran,
Gopal Dharani
Affiliations
Balakrishnan Meena
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair-744103, Andaman and Nicobar Islands, India; Corresponding authors.
Lawrance Anburajan
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair-744103, Andaman and Nicobar Islands, India
Nambali Valsalan Vinithkumar
Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Port Blair-744103, Andaman and Nicobar Islands, India
Ramalingam Kirubagaran
Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai 600100, Tamil Nadu, India
Gopal Dharani
Marine Biotechnology Division, Ocean Science and Technology for Islands Group, National Institute of Ocean Technology, Ministry of Earth Sciences, Government of India, Chennai 600100, Tamil Nadu, India
Trehalose, a non-reducing disaccharide (α-D-glucopyranosyl-(1→1)-α-D-glucopyranoside) is a natural compound, which serves as a protective substance in halophilic bacterial cells. Trehalose biosynthesis genes (otsA and otsB) were PCR amplified from the genomic DNA of deep sea actinobacteria, Streptomyces qinglanensis NIOT-DSA03. The amplified genes were cloned and nucleotide sequences were determined. In silico sequence and phylogenetic analysis of nucleotides and amino acids of otsA and otsB sequences of S. qinglanensis were also determined. The experimental data described in this study will be helpful to develop a recombinant expression system to produce trehalose for biotechnological applications.
