Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana <i>Musa</i> AAA cv. Williams
Fredy P. Carrera,
Carlos Noceda,
María G. Maridueña-Zavala,
José A. García,
Omar Ruiz-Barzola,
Juan M. Cevallos-Cevallos
Affiliations
Fredy P. Carrera
Departamento de Ciencias de la Vida y la Agricultura, Carrera de Agropecuaria, Universidad de las Fuerzas Armadas ESPE, Santo Domingo P.O. Box 171-5-231B, Ecuador
Carlos Noceda
Departamento de Ciencias de la Vida y de la Agricultura, Biología Celular y Molecular de Plantas (BIOCEMP)/Biotecnología Industrial, Universidad de las Fuerzas Armadas-ESPE, Av. General Rumiñahui s/n. Sangolquí, Santo Domingo P.O. Box 171-5-231B, Ecuador
María G. Maridueña-Zavala
Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador CIBE, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, Guayaquil P.O. Box 09-01-5863, Ecuador
José A. García
Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador CIBE, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, Guayaquil P.O. Box 09-01-5863, Ecuador
Omar Ruiz-Barzola
Escuela Superior Politécnica del Litoral, ESPOL, Facultad de Ciencias de la Vida, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, Guayaquil P.O. Box 09-01-5863, Ecuador
Juan M. Cevallos-Cevallos
Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador CIBE, Campus Gustavo Galindo Km. 30.5 Vía Perimetral, Guayaquil P.O. Box 09-01-5863, Ecuador
Micropropagation techniques allow the mass production of banana plants but can cause somaclonal variations such as dwarfism. Changes in the metabolite profile during micropropagation of normal (NP) and dwarf (DP) banana plants have not been described. Both, NPs and DPs of banana Musa AAA cv. Williams were micropropagated and the metabolite profile of vitroplants was assessed at the proliferation (PP), rooting (RP) and the second greenhouse-acclimatization (APII) phases of tissue culture. Metabolites from 10 DPs and 10 NPs meristems from each micropropagation phase were extracted and identified by gas chromatography coupled with mass spectrometry (GC-MS). Principal component analysis (PCA) and test of statistical significance were applied to detect differentially accumulated metabolites. The PCA showed a clear grouping of DPs separated from NPs in RP and APII. Among the differentially accumulated metabolites, various precursors of apoplast components including arabinose and galactose or deoxygalactose in both PP and RP, as well as mannose and fucose in APII were under-accumulated in DPs. Results suggest affected apoplast composition during micropropagation of DPs.
