An Investigation of Antibacterial Activity of ZnO Nanoparticle on Streptoccocus iniae and Escheria coli

Abstract

Background and Objectives: In recent years, in order to prevent excessive use of antibiotics, disinfectant agents have gained particular significance. There are several reports on the antimicrobial effect of ZnO on Gram-positive and Gram-negative bacteria and fungi. In this study, the antibacterial effects of ZnO nanoparticles on Gram-negative bacterium Escherichia coli (because of importance of human health) and Gram-positive bacterium Streptococcus iniae (because of high pathogenicity in fishes), have been investigated. Methods: In this study, minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of this substance were determined using standard liquid dilution and disk diffusion methods for Gram-positive strain (S. iniae) and Gram-negative strain (E. coli) have calculated according to. Data analysis was performed using one-way ANOVA and Duncan tests at the level of p<0/05. Results: According to the results of this study, MBC values of ZnO nanoparticle for S. iniae and E. coli bacteria were calculated to be 0.095 and 0.6µg/ml, respectively, and MIC values for S. iniae and E. coli were calculated 0.015, 0.095µg/ml, respectively. Conclusion: The Results of this study showed that ZnO nanoparticle has acceptable antibacterial and bactericidal effects on S. iniae and E. coli bacteria, and can be used as an antibacterial agent in aqueous environments.

Keywords

• zno nano-particle
• streptococcus
• escherichia coli
• anti -bacterial agents.