Ecotoxicology and Environmental Safety (Sep 2023)
GhCYS2 governs the tolerance against cadmium stress by regulating cell viability and photosynthesis in cotton
- Yuan Meng,
- Yupeng Cui,
- Fanjia Peng,
- Lixue Guo,
- Ruifeng Cui,
- Nan Xu,
- Hui Huang,
- Mingge Han,
- Yapeng Fan,
- Menghao Zhang,
- Yupin Sun,
- Lidong Wang,
- Zhining Yang,
- Mengyue Liu,
- Wenhua Chen,
- Kesong Ni,
- Delong Wang,
- Lanjie Zhao,
- Xuke Lu,
- Xiugui Chen,
- Junjuan Wang,
- Shuai Wang,
- Wuwei Ye
Affiliations
- Yuan Meng
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Yupeng Cui
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Fanjia Peng
- Hunan Institute of Cotton Science, Changde 415101, Hunan, China
- Lixue Guo
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Ruifeng Cui
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Nan Xu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Hui Huang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Mingge Han
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Yapeng Fan
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Menghao Zhang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Yupin Sun
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Lidong Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Zhining Yang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Mengyue Liu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Wenhua Chen
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Kesong Ni
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Delong Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Lanjie Zhao
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Xuke Lu
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Xiugui Chen
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Junjuan Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Shuai Wang
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China
- Wuwei Ye
- Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Research Base, Anyang Institute of Technology, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang 455000, Henan, China; Corresponding author.
- Journal volume & issue
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Vol. 263
p. 115386
Abstract
Cysteine, an early sulfur-containing compound in plants, is of significant importance in sulfur metabolism. CYS encodes cysteine synthetase that further catalyzes cysteine synthesis. In this investigation, CYS genes, identified from genome-wide analysis of Gossypium hirsutum bioinformatically, led to the discovery of GhCYS2 as the pivotal gene responsible for Cd2+ response. The silencing of GhCYS2 through virus-induced gene silencing (VIGS) rendered plants highly susceptible to Cd2+ stress. Silencing GhCYS2 in plants resulted in diminished levels of cysteine and glutathione while leading to the accumulation of MDA and ROS within cells, thereby impeding the regular process of photosynthesis. Consequently, the stomatal aperture of leaves decreased, epidermal cells underwent distortion and deformation, intercellular connections are dramatically disrupted, and fissures manifested between cells. Ultimately, these detrimental effected culminating in plant wilting and a substantial reduction in biomass. The association established between Cd2+ and cysteine in this investigation offered a valuable reference point for further inquiry into the functional and regulatory mechanisms of cysteine synthesis genes.
