Real-time imaging of sulfhydryl single-stranded DNA aggregation

Fanwei Zeng; Youhong Jiang; Nana He; Tiantian Guo; Tiqing Zhao; Mi Qu; Yue Sun; Shuting Chen; Dan Wang; Yong Luo; Guangwen Chu; Jianfeng Chen; Shi-Gang Sun; Hong-Gang Liao

doi:10.1038/s42004-023-00886-6

Communications Chemistry (May 2023)

Real-time imaging of sulfhydryl single-stranded DNA aggregation

Fanwei Zeng,
Youhong Jiang,
Nana He,
Tiantian Guo,
Tiqing Zhao,
Mi Qu,
Yue Sun,
Shuting Chen,
Dan Wang,
Yong Luo,
Guangwen Chu,
Jianfeng Chen,
Shi-Gang Sun,
Hong-Gang Liao

Affiliations

Fanwei Zeng: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Youhong Jiang: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Nana He: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Tiantian Guo: Fujian Provincial Key Laboratory of Neurodegenerative Disease and Aging Research, Institute of Neuroscience, School of Medicine, Xiamen University
Tiqing Zhao: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Mi Qu: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Yue Sun: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Shuting Chen: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Dan Wang: State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology
Yong Luo: State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology
Guangwen Chu: State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology
Jianfeng Chen: State Key Laboratory of Organic-Inorganic Composites and Research Center of the Ministry of Education for High Gravity Engineering and Technology, Beijing University of Chemical Technology
Shi-Gang Sun: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University
Hong-Gang Liao: State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, College of Chemistry and Chemical Engineering, Xiamen University

DOI: https://doi.org/10.1038/s42004-023-00886-6
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 8

Abstract

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Abstract The structure and functionality of biomacromolecules are often regulated by chemical bonds, however, the regulation process and underlying mechanisms have not been well understood. Here, by using in situ liquid-phase transmission electron microscopy (LP-TEM), we explored the function of disulfide bonds during the self-assembly and structural evolution of sulfhydryl single-stranded DNA (SH-ssDNA). Sulfhydryl groups could induce self-assembly of SH-ssDNA into circular DNA containing disulfide bonds (SS-cirDNA). In addition, the disulfide bond interaction triggered the aggregation of two SS-cirDNA macromolecules along with significant structural changes. This visualization strategy provided structure information at nanometer resolution in real time and space, which could benefit future biomacromolecules research.

Published in Communications Chemistry

ISSN: 2399-3669 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry
Website: https://www.nature.com/commschem

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